While Peach and Daisy are seen together in the game's intro, they are not paired together. In Doubles Tournaments, Daisy is Luigi's partner, and Birdo is Daisy's partner. it is the first game to associate Birdo with Daisy.
This is the first game to feature Daisy's modern emblem, which is still used as her emblem today. It can be seen on her tennis racket.
In this game Daisy is shown to be rather clumsy. After winning a game, Daisy spins around and throws her tennis racket upwards, stumbles, and manages to catch it just before it hits the ground. When Daisy wins a tournament, she will show a great deal of excitement and energy before falling off the stage and blushing.
- The game description for the Wii U Virtual Console release of Mario Tennis for the Nintendo 64 refers to Daisy as a fan-favorite character.
- During the development on Mario Tennis on the Nintendo 64, Camelot wanted to create girlfriends for Wario and Waluigi, as Daisy was brought back to be Luigi's girlfriend. This means that "Warupeach" was considered to become a real character. However, Shigeru Miyamoto dismissed the idea.
- This game marks the first official playable appearance of both Daisy and Waluigi.
- This is the first and only game in which Princess Daisy has been voiced by Jessica Chisum.
See the Mario Tennis: Gallery.